Summary: Researchers have developed TRISCO, a cutting-edge imaging method that enables 3D RNA study in total, alive rat brains without slicing them into parts. TRISCO reveals RNA molecules ‘ geographical distribution, providing exceptional insights into the mind’s intricate structure and function.
The strategy will enable more sophisticated studies of brain function and illness to be conducted simultaneously on up to 100 RNA molecules. This development could lead to novel therapies and a new understanding of neurological problems.
Important Facts:
- TRISCO allows three-dimensional RNA scanning in total, alive mouse neurons.
- It may analyze several RNA molecules together, rapidly expanding to 100.
- The approach is appropriate to larger brains as well as larger heart and kidneys tissues.
Origin: Karolinska Institute
A groundbreaking three-dimensional ( 3D ) RNA analysis at cellular resolution in whole intact mouse brains has been achieved by researchers at Karolinska Institutet and Karolinska University Hospital.  ,
The new technique, called TRISCO, has the potential to transform our knowledge of mental work, both in regular conditions and in condition, according to the new research published in , Science.
Despite tremendous advances in RNA evaluation, linking RNA data to its geographical framework has long been a problem, especially in undamaged 3D tissue volumes. The TRISCO technique then eliminates the need to divide whole mouse brains into thin sections, as was earlier required, allowing three-dimensional RNA imaging of entire brains.
This approach has the potential to advance head study. With TRISCO, we can examine the difficult anatomical structure of the brain in a way that was formerly no possible”, says Per Uhlén, professor at the Department of Medical Biochemistry and Biophysics, Karolinska Institutet, and the study’s last writer.
In the study, up to three distinct RNA molecules were examined together. The experts will use a technique known as cinema RNA study to increase the number of RNA particles that can be studied to about a hundred. This might reveal even more in-depth details about disease states and brain function.
The TRISCO approach opens up fresh avenues for examining the degree of brain complexity, which in turn could lead to the development of novel treatments for different brain conditions.
Shigeaki Kanatani, a research consultant in Uhlén’s lab and the study’s second author,” We look forward to continuing our studies and exploring the many opportunities that this new technique has.”
Not only is TRISCO suitable for studying alive mouse brains, but the research demonstrates it can be used for larger neurons, such as those of guinea pigs, and various tissues like liver, brain, and heart. Karolinska Institutet and Karolinska University Hospital collaborate on this study.
” Our laboratory collaborates with Karolinska University Hospital’s clinically active researchers in a number of ways.” Basic researchers and clinicians must work together and comprehend one another, according to Per Uhlén.
Funding: The study has been funded by the Swedish Research Council, Swedish Brain Foundation and Swedish Cancer Society. Some of the co-authors work for and own Gubra, a Danish company. No other conflicts of interest are reported.
About this news story about RNA and neuroimaging research
Author: Press Office
Source: Karolinska Institute
Contact: Press Office – Karolinska Institute
Image: The image is credited to Science
Original Research: Closed access.
” Whole-Brain Spatial Transcriptional Analysis at Cellular Resolution” by Per Uhlén et al. Science
Abstract
Whole-Brain Spatial Transcriptional Analysis at Cellular Resolution
Our understanding of cellular states in biological tissues has expanded as a result of recent advances in RNA analysis. Despite the difficulties that come with integrating RNA expression data with spatial context across organs, a significant gap still exists, primarily because of the difficulties that come with RNA detection inside intact tissue volumes.
Here, we developed Tris buffer–mediated retention of in situ hybridization chain reaction signal in cleared organs ( TRISCO ), an effective tissue-clearing method designed for whole-brain spatial three-dimensional ( 3D ) RNA imaging.
TRISCO resolved several crucial issues, including the preservation of RNA integrity, achieving uniform RNA labeling, and enhancing tissue transparency.
We tested TRISCO using a broad range of cell-identity markers, noncoding and activity-dependent RNAs, within diverse organs of varying sizes and species.
TRISCO thus emerges as a powerful tool for single-cell, whole-brain, 3D imaging that enables comprehensive transcriptional spatial analysis across the entire brain.
